The isolates were cultured on bordetgengou agar difco or cyclodextrin solid medium csm agar 31, and incubated at 36uc for 223 days. Primarily used for observing hemolytic reactions produced by many species of bacteria. Bordet gengou agar base bordet gengou blood agar intended use bordet gengou agar base, with the addition of glycerol and sterile blood, is used in qualitative procedures for the detection. Directions aseptically add 2ml of sterile distilled water to one vial and mix gently to dissolve the contents completely. Bordet gengou agar media were originally formulated by bordet and gengou 1 for cultivation of bordetella species. Fieldinoculated plates of reganlowe agar medium were most useful if incubation was initiated on the. Remel bordet gengou agar base thermo fisher scientific. Method of preparation method of preparation varies depending on the application. Pdf comparison of bloodfree medium cyclodextrin solid. It is available in the form of fine, light colored granules which are convenient for weighing and handling.
Shop online for a wide selection of thermo scientific remel bordet gengou agar with blood with blood. Defibrinated sheep blood supplies additional nutrients and enables the. Aseptically add the contents to 500ml of sterile molten charcoal agar cm0119, cooled to approximately 50c, together with 10% defibrinated horse blood sr0050. Bordetella pertussis is the causative agent of whooping cough and with the help of coughplate technique, b. Pdf cyclodextrin solid medium csm developed by us was evaluated to be a. Bordet gengou agar base, with the addition of glycerol and. Thermo scientific remel bordet gengou agar with blood. Bd bordet gengou agar with 15% sheep blood bd bordetella. Defibrinated animal blood supplies additional nutrients and enables the detection of hemolytic reactions, which aid in the identification of b. Difco agar difco broth and all difco dehydrated media shipped worldwide. Difco microbiology media, difco antigens, difco antiserum and additives and all allied difco reagents. Specimen collection and preparation obtain and process specimens according to the techniques and. Bordetgengou agar is a type of agar plate optimized to isolate bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or. Rehydrate pellets of freezedried ampoules in liquid medium 220 or 1.
A solidifying agent, is a purified agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. Bordet gengou agar base bordet gengou blood agar intended use bordet gengou agar base, with the addition of glycerol and sterile blood, is used in qualitative procedures for the detection and isolation of bordetella pertussis from clinical specimens. Enumeration of coliforms in water by the membrane filter technique. Remel bordet gengou agar base is used for the isolation of bordetella pertussis and bordetella parapertussis. Bacto agar agar flake agar, granulated agar noble agar bacteriological technical materials required but not provided materials vary depending on the application. Bordet gengou blood agar contains potato infusion and glycerol to supply the nutrients necessary to support the growth of b. Bordet gengou agar base difco 248200 worldwide shipping and reseller discounts are available to qualified customers. Food testing for staphylococcus aureus using bbl chromagar staph aureus. Western blotting detection system, according to the manufactures instruction. Used with the addition of gycerol and blood for the isolation of bordetella pertussis and bordetella parapertussis. Prepared without carbohydrates for coliform fermentation studies. Enriched with defibrinated blood for the isolation of b.
Bordet gengou agar is a type of agar plate optimized to isolate bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide. Bordetgengou medium difco bordet gengou agar base supplemented with 15% horse blood. Summary and explanation the enterococcus group is a subgroup of the fecal strepto. Manual of microbiological culture media becton dickinson. Presterilized tubes of bg base, however, can bestored for 1.
The isolates were cultured on bordetgengou agar difco or cyclodextrin solid. Meets united states pharmacopeia usp, european pharmacopoeia ep and japanese pharmacopoeia jp performance specifications, where applicable. Summary and explanation bordet gengou blood agar is used in clinical laboratories as a. Lactobacilli mrs agar is part of a large selection of dehydrated media.
Bordetgengou potato blood agar definition of bordet. Macconkey agar base is part of a large selection of dehydrated media. As a trusted supplier of a broad range of high quality media and diagnostic products used in clinical, industrial, research, and academic laboratories, and backed by a team of service experts, we are dedicated to microbiology. Bd difco microbiology difco culture media bile esculin agar difco 299068 500g. Comparison of modified bordetgengou and modified reganlowe. Orders media by organism bordetella pertussis parapertussis bordet gengou agar base bordet gengou agar base. Bordetgengou agar definition of bordetgengou agar by. Difco bordet gengou agar base becton, dickinson and company, catalog. Bd diagnostic systems regulatory regulatory documents. Use a sterile scalpel or needle to remove the portions. Noble agar is perfect for electrophoretic and nutritional procedures wherever increased purity is required.
It is usually used in concentrations of 12% in solid culture media or in concentrations of 0. Bordet gengou agar base is a modification of the medium originally described by bordet and gengou for the cultivation of bordetella pertussis. Bbl difco laboratories maintains a strong commitment to research and development, resulting in an extensive range of media formulations. Summary and explanation ym agar and ym broth are prepared according to the formulae published by wickerham. Bd is a leading medical technology company that advances human health in every corner of the world by improving medical research, diagnostics and the delivery of care. Used as a solidifying agent essentially free of impurities. A growth medium used in microbiology labs to isolate bordetella pertussis and b parapertussis. Difco egg yolk enrichment 50% is a concentrated egg yolk emulsion recommended for use in a variety of media such as difco sfp agar base and difco mcclung toabe agar base for the isolation and identification of clostridium species on the basis of their lecithinase activity. Bordet gengou blood agar is an enriched, casein peptone medium with potato infusion and glycerol to supply the nutrients necessary to support the growth of 8. The colonies are difco bordet gengou agar base usually. For cultivation and isolation of bordetella pertussis and other. Bordetgengou potato blood agar definition of bordetgengou. Manufactured and tested for uniformity and consistency from lottolot. The isolates were cultured on bordet gengou agar difco or cyclodextrin solid medium csm agar 31, and incubated at 36uc for 223 days.